Correlation of the “EMIT” with a Gas-Liquid Chromatographic Method for Determination of Antiepileptic Drugs in Plasma

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Abstract
We report a study of the comparison of antiepileptic drugs (pheno-barbital, phenytoin, primidone, and carbarnazepine) by the Enzyme Multiplied Immunoassay Technique “EMIT” and the gas chromatography (GLC) method. Overall, reasonable correlations were observed for all of the above-mentioned assays by both methods in the majority of samples included in this study. Our observations for statistical and clinical differences at various levels of phenobarbital and phenytoin are discussed. A suggestion is provided in order to avoid a major discrepancy (approximately 30% higher values by EMIT vs GLC) in results observed from the “Bottom of the Bottle Effect” for EMIT reagent.