Carboxypeptidase in Mammalian Tissue.

Abstract
Using chloracetyl-L-tyrosine as substrate, carboxypeptidase was found widely distr. through mammalian tissues. Previous failures to detect the enzyme are due to: (a) the existence of a natural cellular inhibitor, which masks the enzyme; and (b)the enzyme probably exists in 2 forms. The pH optima of these 2 forms are different, and one is activated, the other inhibited, by cysteine. The natural inhibitor is protein in nature, affects only one of the forms and in a non- competitive fashion. The enzymatic conversion of one form of the enzyme to the other was detected in vitro.
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