Apparatus to measure fluorescence decay curves

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Abstract
A pulse fluorimeter is described which allows measurements of fluorescence decays in the range 1 ns to 1 μs. The system is built from a modified Hundley light source with 1 kHz repetition frequency and a commercially available sampling oscilloscope combined with a signal averager. The signal is digitized at 254 discrete time positions with a 10 bit resolution. Up to 256 scans can be summed and the data can be read out either analogue or on punched tape for digital processing. Both the shape of exitation pulse and the fluorescence decay curve are measured and individually corrected for spurious signals.

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