The Effect of Adenosine Triphosphate on Phosphoglycerate Mutase Activity from Hyphomicrobium x and Pseudomonas AM1 Grown on Reduced One-carbon Compounds
- 1 December 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 97 (2) , 335-338
- https://doi.org/10.1099/00221287-97-2-335
Abstract
The effect of AMP, ADP and ATP on the activity of phosphoglycerate mutase, EC 2.7.5.3, in crude cell-free extracts, partially purified enzyme preparations and purified enzyme preparations from Hyphomicrobium X and Pseudomonas AM1 was studied. Added ATP stimulated activity in both extracts, and the stimulation was antagonized by AMP and ADP. The nucleotides did not affect the enzyme activity of partially purified and purified enzyme preparations. Thus, the initial stimulatory effect was probably an artefact due to interference of other enzymes present in the crude extracts during the assay procedure.This publication has 2 references indexed in Scilit:
- Purification and Characterization of Phosphoglycerate Mutase from Methanol-grown Hyphomicrobium x and Pseudomonas AM1Journal of General Microbiology, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951