Nucleic acid (cDNA) and amino acid sequences of isocitrate lyase from castor bean
- 1 January 1987
- journal article
- conference paper
- Published by Springer Nature in Plant Molecular Biology
- Vol. 8 (6) , 471-475
- https://doi.org/10.1007/bf00017992
Abstract
A cDNA library was constructed to mRNA enriched for isocitrate-lyase mRNA from castor-bean (Ricinus communis var. zanzibarensis) endosperms. Nine clones for isocitrate lyase (EC 4.1.3.1) were identified. The insert of 2.2 kb from clone ICL4 was sequenced and proved to contain the entire coding region, 1731 bp, for isocitrate lyase. The amino acid sequence of isocitrate lyase was deduced from the nucleic acid sequence. By analogy with muscle aldolase a lysine residue that possibly takes part in the binding of the substrate was identified. The 3′ untranslated region contained three putative polyadenylation addition signals and two direct repeats.This publication has 9 references indexed in Scilit:
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