A histochemical method to identify the genotype of single embryo cultures of Drosophila melanogaster.

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Abstract
In the developmental analyses of Drosophila mutants, it is necessary to distinguish mutant embryos from heterozygous or normal ones. An enzyme marker system was developed by means of an X-linked mutation Zwischenfermentn (Zwn). By histochemical staining for the activity of glucose-6-phosphate dehydrogenase (G6PD, E.C.1.1.1.49) in a single embryo culture, Zwn cultures were distinguishable from Zw+ ones. The applicability of the method to the analysis of the X-linked developmental lethals was demonstrated by examining the cell differentiation in vitro of an embryonic lethal mutation l(1)EI43. A morphological abnormality was found specifically in the cultures from hemizygous lethal embryos.