ELISA DETECTION OF IGM ANTIBODIES AGAINST PHENOLIC GLYCOLIPID-I IN THE MANAGEMENT OF LEPROSY - A COMPARISON BETWEEN LABORATORIES
- 1 December 1986
- journal article
- research article
- Vol. 54 (4) , 530-539
Abstract
IgM antibodies to the phenolic glycolipid-I (PGL-I) antigen of Mycobacterium leprae were detected by different ELISA techniques in three laboratories (in New York, Colorado, Seattle, USA). The agreement on seropositivity and overall correlation between techniques was excellent. A positive linear correlation between the bacterial index (BI) and anti-PGL-I IgM, previously reported by the New York laboratory, was detected by all techniques. The role of erythema nodosum leprosum in decreasing the relationship of BI versus anti-PGL-I IgM was seen by the New York laboratory with sera diluted 1:20 and ABTS substrate solution and by the Colorado laboratory but not by New York with sera at 1:300 and OPD substrate or by the Seattle laboratory.This publication has 10 references indexed in Scilit:
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