First Outbreak of Respiratory Disease Associated with Swine Influenza H1N2 Virus in Pigs in Korea
Open Access
- 1 March 2005
- journal article
- research article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 17 (2) , 176-178
- https://doi.org/10.1177/104063870501700213
Abstract
This report describes the first diagnosis of swine influenza H1N2 virus infection in growing pigs in Korea by virus isolation, reverse transcription–polymerase chain reaction (RT-PCR), histopathology, and in situ hybridization. The subtype of swine influenza virus isolates was determined to be H1N2 by RT-PCR. The most consistent and predominant histological feature was bronchointerstitial pneumonia. Lung tissues from these pigs were hybridized with the nonradioactive digoxigenin-labeled complementary DNA (cDNA) probe from H1 HA and N2 NA genes but not from H3 HA and N1 NA genes. A strong hybridization signal was seen in bronchial- and bronchiolar-lining epithelial cells and in alveolar and interstitial macrophages.Keywords
This publication has 14 references indexed in Scilit:
- Detection and subtyping of swine influenza H1N1, H1N2 and H3N2 viruses in clinical samples using two multiplex RT-PCR assaysJournal of Virological Methods, 2002
- Evaluation of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for Subtyping Hemagglutinin Genes 1 and 3 of Swine Influenza Type A Virus in Clinical SamplesJournal of Veterinary Diagnostic Investigation, 2002
- Differentiation of porcine circovirus 1 and 2 in formalin-fixed, paraffin-wax-embedded tissues from pigs with postweaning multisystemic wasting syndrome by in-situ hybridisationResearch in Veterinary Science, 2001
- Distribution of a Korean Strain of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected Pigs, as Demonstrated Immunohistochemically and by In-situ HybridizationJournal of Comparative Pathology, 1999
- Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H1N2 virus of novel genotype.Journal of General Virology, 1998
- Large outbreak of swine influenza in southern Japan caused by reassortant (H1N2) influenza viruses: its epizootic background and characterization of the causative virusesJournal of General Virology, 1996
- Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtypeVeterinary Record, 1995
- Isolation of two H1N2 influenza viruses from swine in FranceArchiv für die gesamte Virusforschung, 1994
- The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 virusesVirology, 1985
- Further Isolation of a Recombinant Virus (H1N2, Formerly Hsw1N2) from a Pig in Japan in 1980Microbiology and Immunology, 1983