Hb regina or α2β296(FG3)LEU → VAL: A high oxygen affinity variant discovered by cation‐exchange HPLC
- 1 June 1985
- journal article
- research article
- Published by Wiley in American Journal of Hematology
- Vol. 19 (2) , 195-200
- https://doi.org/10.1002/ajh.2830190212
Abstract
Results are reported of studies of the hemoglobin from one member of a Canadian family with a mild erythrocytosis. This variant, which accounted for about 40% of the hemoglobin, could be separated from Hb A and Hb A2 by cation‐exchange chromatography. Micro methodology allowed the characterization of a Leu → Val replacement at position β96(FG3). Hb Regina has an increased oxygen affinity, which adequately explains the hematological observations.Keywords
This publication has 17 references indexed in Scilit:
- Quantitation of hemoglobin components by high‐performance cation‐exchange liquid chromatography: Its use in diagnosis and in the assessment of cellular distribution of hemoglobin variantsAmerican Journal of Hematology, 1984
- A Silent, Neutral Substitution Detected by Reverse-Phase High-Performance Liquid Chromatography: Hemoglobin BeirutScience, 1983
- Hemoglobin pitie-salpetriere β34 (B16) Val → Phe a new high oxygen affinity variant associated with familial erythrocytosisBiochimica et Biophysica Acta (BBA) - Protein Structure, 1980
- Separation of tryptic peptides of normal and abnormal a, β, γ, and δ hemoglobin chains by high-performance liquid chromatographyJournal of Chromatography A, 1979
- Micro‐sequence analysis of peptides and proteins using 4‐NN‐dimethylaminoazobenzene 4′‐isothiocyanate/phenylisothiocyanate double coupling methodFEBS Letters, 1978
- Stereochemistry of Cooperative Effects in Haemoglobin: Haem–Haem Interaction and the Problem of AllosteryNature, 1970
- The Croonian Lecture, 1968 - The haemoglobin moleculeProceedings of the Royal Society of London. B. Biological Sciences, 1969
- Three-dimensional Fourier Synthesis of Horse Oxyhaemoglobin at 2.8 Å Resolution: The Atomic ModelNature, 1968
- Abnormal human haemoglobins: Separation and characterization of the α and β chains by chromatography, and the determination of two new variants, Hb chesapeake and Hb J (Bangkok)Journal of Molecular Biology, 1966
- Structural Studies of Aminoethylated Hemoglobins by Automatic Peptide ChromatographyCold Spring Harbor Symposia on Quantitative Biology, 1964