Säurehydrolyse von Ribonucleinsäure und Desoxyribonucleinsäure
Open Access
- 1 October 1961
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung B
- Vol. 16 (10) , 673-678
- https://doi.org/10.1515/znb-1961-1007
Abstract
A method for the potentiometric titration of secondary phosphate groups in nucleic acids is described. Ribonucleic acids of yeast and of microsomes contain 5—6% secondary phosphate groups which cannot be removed by dialysis. The potentiometric method was applied to study several enzymatic hydrolyses and the non-enzymatic hydrolysis between pH 2.4 —1.8. The rate of hydrolysis for the purines and for the phosphate groups is approximately proportional to the H®-concentration. The constants of hydrolysis for ribonucleic acid and for deoxyribonucleic acid were determined. In DNA the depurinisation is 650 times faster than in RNA.Keywords
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