Selective Plating Medium for Leuconostoc in Mixed Lactic Cultures

Abstract
Leuconostoc was found to be more resistant than Streptococcus to small amounts of tetracycline hydrochloride. Consequently a plating medium for detecting and enumerating leuconostocs in multiple strain starters has been developed. It contains a tetracycline concentration of .15[mu]g/ml in a tomato juice agar base. Five experimentally mixed starters of known composition (Streptococcus and Leuconostoc) and 7 commercial multiple strain lactic starters were used to evaluate the medium. After incubation at 30oC for 48 hr, 99% of representative isolates were identified as Leuconostoc.

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