Humanized NOD/SCID/IL2RγnullMice Transplanted with Hematopoietic Stem Cells under Nonmyeloablative Conditions Show Prolonged Life Spans and Allow Detailed Analysis of Human Immunodeficiency Virus Type 1 Pathogenesis
Open Access
- 1 December 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (23) , 13259-13264
- https://doi.org/10.1128/jvi.01353-07
Abstract
In a previous study, we demonstrated that humanized NOD/SCID/IL2Rγnull (hNOG) mice constructed with human hematopoietic stem cells (HSCs) allow efficient human immunodeficiency virus type 1 (HIV-1) infection. However, HIV-1 infection could be monitored for only 43 days in the animals due to their short life spans. By transplanting HSCs without any myeloablation methods, the mice successfully survived longer than 300 days with stable engraftment of human cells. The mice showed high viremia state for more than the 3 months examined, with systemic HIV-1 infection and gradual decrease of CD4+ T cells analogous to that in humans. These capacities of the hNOG mice are very attractive for modeling mechanisms of AIDS progression and therapeutic strategy.Keywords
This publication has 35 references indexed in Scilit:
- Human Immunodeficiency Virus Type 1 Pathobiology Studied in Humanized BALB/c-Rag2 −/− γ c −/− MiceJournal of Virology, 2007
- HIV-1 infection and pathogenesis in a novel humanized mouse modelBlood, 2006
- Disseminated and sustained HIV infection in CD34+cord blood cell-transplanted Rag2−/−γc−/−miceProceedings of the National Academy of Sciences, 2006
- Functional CD5+ B cells develop predominantly in the spleen of NOD/SCID/γcnull (NOG) mice transplanted either with human umbilical cord blood, bone marrow, or mobilized peripheral blood CD34+ cellsExperimental Hematology, 2003
- Complete reconstitution of human lymphocytes from cord blood CD34+ cells using the NOD/SCID/γcnull mice modelBlood, 2003
- Hematopoietic Repopulating Ability of Cord Blood CD34+Cells in NOD/Shi‐scidMiceThe International Journal of Cell Cloning, 2000
- Rapid Loss of CD4 + T Cells in Human-PBL-SCID Mice by Noncytopathic HIV IsolatesScience, 1993
- The SCID-hu Mouse: A Small Animal Model for HIV Infection and PathogenesisAnnual Review of Immunology, 1991
- Human Immunodeficiency Virus Infection of Human-PBL-SCID MiceScience, 1991
- Infection of the SCID-hu Mouse by HIV-1Science, 1988