Extractive bioconversions in aqueous two‐phase systems: Enzymatic hydrolysis of casein proteins
- 20 June 1992
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 40 (2) , 195-206
- https://doi.org/10.1002/bit.260400202
Abstract
Partition coefficients in poly(ethylene glycol)/dextran aqueous two‐phase systems are reported for mixed‐casein and its components, α, β and κ casein. Rates of casein proteolysis by α‐chymotrypsin and by trypsin are reported in single‐phase and aqueous two‐phase reactor systems. The advantages resulting from selective partitioning of substrates, enzymes, and products are examined in terms of relative volumetric reaction rates.Keywords
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