Chemical Contrast in X-Ray Microscopy and Spatially Resolved XANES Spectroscopy of Organic Specimens
- 6 November 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 258 (5084) , 972-975
- https://doi.org/10.1126/science.1439809
Abstract
The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.Keywords
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