Nucleotide sequence and organization of potato leafroll virus genomic RNA

Abstract
The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA‐dependent RNA polymerase (70 kDa) were identified by interviral amino acid sequence homologies. For expression of the different ORFS, translational frameshift and readthrough events are proposed.