Idiotope‐specific T cell clones that recognize syngeneic immunoglobulin fragments in the context of class II molecules

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Abstract
We have established idiotope (Id)-specific T cell lines and clones derived from at least 4 different BALB/c mice immunized with the light chain (λ2315) of the BALB/c myeloma protein M315 (α,λ2). Independently derived clones were indistinguishable in that they reacted to Vλ2315, one or more of the amino acids corresponding to somatically mutated codons 94, 95 and 96 of the third hypervariable region being essential for expression of the Id. While the Id was efficiently expressed on Vλ2315, Fv315 and λ2315 fragments, about a 100–1000-fold higher molar concentration of Fab315and M315 was needed to induce equivalent responses. Thus, Ig quaternary structure heavily influenced the availability of the Id for T cells. The Vλ2315-specific T cells were Thy-1.2+, L3T4+, Ly-2.2− and I-Ed restricted. Some of the T cell clones produced interleukin 2 (IL2), IL3 and B cell growth and differentiation factors upon activation. In addition, T cells were cytotoxic in long-term assays for EE, but not EE-transfected L cells in the presence of Id. The cytotoxic effect was the basis for an L cell growth inhibition assay for T cell activation that was at least 10-fold more sensitive than ordinary proliferation assays.

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