Monolayer Tissue Cultures
Open Access
- 1 December 1954
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 73 (6) , 392-396
- https://doi.org/10.4049/jimmunol.73.6.392
Abstract
Summary: The influence of the composition of the medium employed and the time of fluid change on the number of cells comprising the cell monolayer have been investigated and growth curves for poliomyelitis virus in this culture system have been determined. The results presented indicate that the use of trypsin-dispersed monkey kidney cell suspensions yielded fluids with infectivity titers of 107.3 to 108.2 ID50 doses per ml.Keywords
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