TO THE KNOWLEDGE OF HUMAN PORINS .1. PURIFICATION OF A PORIN FROM HUMAN B-LYMPHOCYTES (PORIN 31 HL) AND THE TOPOCHEMICAL PROOF OF ITS EXPRESSION ON THE PLASMALEMMA OF THE PARENTAGE CELL-LINE
- 1 December 1989
- journal article
- research article
- Vol. 370 (12) , 1253-1264
Abstract
We describe for the first time a porin (Porin 31HL) on the plasmalemm of an eukaryotic cell line, where porins have been found only on the outer mitochondrial membranes. The expression of the porin on the plasmalemm of transformed human B-lymphocytes is demonstrated by cytotoxicity- and indirect immunoflourescence techniques with living and fixed cells. The rabbit xenoantisera used were directed against purified Porin 31HL and free or acetylated synthetic peptides of its nineteen N-terminal amino acids. The three-step purification procedure for Porin 31HL started from a total membrane fraction of the B-cell line, followed by ion-exchange chromatography on CM- and DEAE-cellulose and a final gel filtration in SDS on Sephacryl S-300.This publication has 19 references indexed in Scilit:
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