The Effects of PEG on Second Antibody Immunoprecipitation and Its Use in Immunoassay
- 1 December 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 14 (4) , 241-266
- https://doi.org/10.1080/15321819308019853
Abstract
The effects of PEG on the second antibody immunoprecipitation have been studied in several radioimmunological systems. The following parameters have been studied in the assays: the average molecular weight and concentration of PEG, the species of the animal producing the first and second antibody, the nature of the antigen, the concentration of carrier immunoglobulins, and the rate of the immunoprecipitation. Thus, optimal conditions for the use of PEG with a second antibody have been defined in order to be used in any liquid phase immunoassay.Keywords
This publication has 6 references indexed in Scilit:
- Early mouse molar root development: Cellular changes and distribution of fibronectin, laminin and type-IV collagenDifferentiation, 1985
- Ser-Leu-Arg-Arg-Atriopeptin III: The Major Circulating Form of Atrial PeptideScience, 1985
- Synthesis in vitro of precursors of the catalytic subunits of acetylcholinesterase from Torpedo marmorata and Electrophorus electricusEuropean Journal of Biochemistry, 1984
- Relationship of Collagen‐Tailed Acetylcholinesterase with Basal Lamina ComponentsEuropean Journal of Biochemistry, 1983
- [2] Production of antisera with small doses of immunogen: multiple intradermal injectionsPublished by Elsevier ,1981
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962