Polymerase Chain Reaction‐Restriction Fragment Length Polymorphisms (PCR‐RFLP) and Electrophoretic Assays for the Mouse Obese (Lepob) Mutation
Open Access
- 1 May 1997
- journal article
- research article
- Published by Wiley in Obesity Research
- Vol. 5 (3) , 183-185
- https://doi.org/10.1002/j.1550-8528.1997.tb00291.x
Abstract
Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C←T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A←C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the hetero-zygote.Keywords
This publication has 8 references indexed in Scilit:
- Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptinNature Genetics, 1996
- Decreased food intake does not completely account for adiposity reduction after ob protein infusion.Proceedings of the National Academy of Sciences, 1996
- Recombinant ob protein reduces feeding and body weight in the ob/ob mouse.Journal of Clinical Investigation, 1995
- Recombinant Mouse OB Protein: Evidence for a Peripheral Signal Linking Adiposity and Central Neural NetworksScience, 1995
- Weight-Reducing Effects of the Plasma Protein Encoded by the obese GeneScience, 1995
- Effects of the obese Gene Product on Body Weight Regulation in ob / ob MiceScience, 1995
- Positional cloning of the mouse obese gene and its human homologueNature, 1994
- Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP)Nucleic Acids Research, 1994