Introduction of Bacteriophage Mu into Erwinia stewartii by Use of an RK2:: Mu Hybrid Plasmid
- 1 July 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 113 (1) , 181-184
- https://doi.org/10.1099/00221287-113-1-181
Abstract
Although bacteriophage Mu cannot infect E. stewartii, lysogens were constructed by conjugal transfer of an RK2::Mu cts 62 hybrid plasmid, pRK210, into this species from Escherichia coli. The pRK210 transconjugants were stable and produced infectious Mu virions. The Mu cts 62 prophage was thermally induced at 42.degree. C and zygotically induced following transfer of the plasmid to E. stewartii recipients. Induction was accompanied by an increased frequency of Gal- and Thy- mutants among the survivors. Mu promises to be an important tool for studying the genetics of pathogenicity in E. stewartii.This publication has 5 references indexed in Scilit:
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