Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes
- 1 January 1986
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 6 (1) , 39-44
- https://doi.org/10.1007/bf01145177
Abstract
A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubitized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of detergent (below 0.001%) and is inhibited by native peptide. A molecular weight of about 80,000 was estimated for VIP-binding proteins by reference to a series of globular markers of proteins. Binding to VIP soluble proteins is specific and dependent on time as studied by the Hummel and Dreyer (Biochim. Biophys. Acta63:530–532, 1962) assay.This publication has 22 references indexed in Scilit:
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