Hypochlorite Disinfection Influences the GA3-Induced Synthesis of α-Amylase Isozymes by Isolated Barley Aleurone Layers
- 1 February 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 86 (2) , 510-511
- https://doi.org/10.1104/pp.86.2.510
Abstract
Aleurone layers isolated from half-seeds of Himalaya barley (Hordeum vulgare cv Himalaya) disinfected in hypochlorite solutions containing 1.0% available chlorine synthesized significantly less .alpha.-amylase in response to gibberellic acid than layers derived from half-seeds disinfected in 0.1% hypochlorite. This effect of hypochlorite involved neither a differential decrease in the synthesis of group A or B .alpha.-amylase isozymes nor a general decrease in .alpha.-amylase synthesis attributable to fewer viable aleurone cells in layers from half-seeds disinfected with 1% hypochlorite. Our results emphasize the need to evaluate the potential effects of routine disinfection procedures used in physiological and biochemical studies.This publication has 5 references indexed in Scilit:
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