Biochemical and Morphological Studies of Rat Submandibular Gland: I. Centrifugal Fractionation of Granule-Rich Fraction
- 1 September 1975
- journal article
- Published by SAGE Publications in Journal of Dental Research
- Vol. 54 (5) , 938-947
- https://doi.org/10.1177/00220345750540053201
Abstract
Submandibular glands from male rats were homogenized in 0.34 M sucrose and 0.5 mM ethylenediaminetetraacetic acid in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through nuclepore filters to prepare a granule-rich fraction. Electron dense zymogen granules constituted approximately 85% of the particles in this fraction which also contained about a third of the total alkaline esterase activity in the gland.Keywords
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