Application of the Altmann Freezing-Drying Technique to Plant Cytology
- 1 September 1934
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 20 (9) , 495-501
- https://doi.org/10.1073/pnas.20.9.495
Abstract
The Altmann technique of fixation by dehydrating frozen tissues in a vacuum at -20[degree]C. or lower was applied to anthers of Lilium longiflorum and root tips of Allium cepa, employing mechanical refrigeration. Theory regarding dehydration process shows vacuum pressure need not be lower than 1 micron of Hg, at -32[degree]C. A convenient apparatus is described. Plant tissue, as contrasted with certain rat tissues, required a longer time for both dehydration and paraffin infiltration and gave in general a lower quality of fixation. However, the method often rendered unusually clear the mitochondria and chromonematic details of chromosomes, the latter being further discussed in a later article (Univ. California Publ. Bot. 18(2/3) 23-44. 1935).This publication has 1 reference indexed in Scilit:
- The Altmann technique for fixation by drying while freezingThe Anatomical Record, 1932