Fate ofd- andl-alanine fed to rice leaf blades

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Abstract
D- and l-[1-14C]alanine were fed under identical conditions to excised rice leaf blades and the distribution patterns of radioactivity in the tissues were compared. The radioactivity in ethanol extracts after feeding l-alanine decreased rapidly with time, whereas that of d-alanine decreased slowly. The percent of radioactivity in the cationic fraction of ethanol extracts of leaf blades fed with d-alanine was considerably higher than that in the case of l-alanine. In the cationic fraction fed with d-alanine, an unknown compound (I) and d-alanylglycine were labeled predominantly and the labeled positions in these two compounds were an alanine moiety. Labeled alanine was also detected in the non-cationic fraction of ethanol extracts of leaf blades fed with d-alanine. The configuration of the labeled alanine was the d-isomer. These findings indicate that exogenous d-alanine was transferred to conjugated d-alanine with retention of configuration. On the other hand, after feeding l-alanine, such a labeled compound was not detected in either the cationic or non-cationic fraction insofar as determinations by paper radiochromatography were concerned.

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