Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes
- 1 February 1991
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology – Animal
- Vol. 27 (2) , 137-141
- https://doi.org/10.1007/bf02630999
Abstract
We have investigated the regulation of the two normal differentiation pathways followed by laryngeal epithelium. Using a tissue culture system that permits growth of cells at the air-liquid interface in serum-free medium, we found that modulating the concentration of retinoic acid is sufficient to determine which pathway is used. At 10−8 M retinoic acid, the cells form a stratified squamous epithelium which expresses the differentiation-specific keratin K13. At 10−7 M retinoic acid, the cells form a ciliated pseudostratified epithelium, with no expression of K13. These results are distinct from those seen with foreskin keratinocytes, which have only a single pathway of normal differentiation.Keywords
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