Alternative Method for Analyzing Protein Characters in Lichens

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Abstract
A number of techniques were tried for disrupting lichen cells mechanically. The most effective was to pulverize acetone-washed thallus fragments in liquid nitrogen. For separation of lichen proteins, isoelectricfocussing on polyacrylamide gel was superior to electrophoresis. The proteins were visualized using Coumassie Brilliant Blue, and the isoelectric points of most were .apprx. 4.0-6.5. [This method was appropriate for routine use in lichen systematics.].

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