Modulation of large conductance Ca 2+ -activated K + channel by Gα h (transglutaminase II) in the vascular smooth muscle cell
- 24 February 1997
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 433 (5) , 671-673
- https://doi.org/10.1007/s004240050330
Abstract
Among G-proteins, Gh is unique in structural differences in the GTP-binding domain and possessing transglutaminase activity. We have studied the role of G protein in modulation of large conductance Ca2+-activated K+ (Maxi-K+) channel by the inside-out mode of patch clamp in smooth muscle cells from superior mesenteric artery of the rabbit. When the non-hydrolyzable GTP analogue, GTPγS, was applied, the channel activity was increased about 2.5-fold. Addition of GDPβS resulted in reversal of the GTPγS effect. When the Gαh7 antibody was applied, the GTPγS-stimulated channel activity was significantly inhibited to control level, suggesting that Gαh is involved in activation of the Maxi-K+ channel in smooth muscle cells.Keywords
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