Staining of biomembranes with amphiphilic hemicyanine dyes

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Abstract
The binding of amphiphilic dyes to biomembranes is described as a distribution equilibrium between a three‐dimensional and a two‐dimensional phase. Ostwald‐type coefficients and formal binding constants are measured for lipid vesicles and hemicyanine dyes with different hydrophilic‐hydrophobic balance. The staining of neuron membranes is discussed as a basis for optical recording of membrane voltage by hemicyanine dyes.