Production of Single-walled Cysts of Schizopyrenus russelli by Sonication and their Behaviour Towards Excystment-inducing and Excystment-blocking Agents
Open Access
- 1 March 1970
- journal article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 60 (3) , 387-392
- https://doi.org/10.1099/00221287-60-3-387
Abstract
SUMMARY: Ultrasonic treatment of double-walled cysts of Schizopyrenus russelli led to the removal of the outer wall. These single-walled cysts excysted readily in aqueous extract of Escherichia coli, as did normal double-walled cysts. There was a marked delay in the excystment of single-walled cysts obtained by ultrasonic treatment of the normal cysts treated with p-chloromercuric benzoate (p-CMB) or emetine, similar to that obtained with normal cysts. The action of p-CMB was annulled by reduced glutathione. These findings suggest that the sites of action of excystment-inducing and excystment-blocking agents are on or inside the inner cyst wall and that these agents are able to permeate the outer cyst wall.Keywords
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