ANTIGEN-SPECIFIC MAST-CELL DE-GRANULATION IN CONTACT SENSITIVITY TO PICRYL CHLORIDE - EARLY EVENT

  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (3) , 331-336
Abstract
Mast cells from the peritoneum of mice painted with the contact sensitizing agent picryl chloride degranulate when exposed to antigen (TNP [trinitrophenyl]) in vitro. Degranulation was consistently demonstrated 4 days after painting which associated with the ability of mice to produce contact sensitivity reactions as measured by ear swelling and radiometric assays. Serum reagin or reagin-producing cells could not be detected until 6 days after painting, but TNP-phage neutralizing activity was detected after 2 days. Mast cell degranulation could be elicited by TNP or DNP [dinitrophenyl] derivatives, indicating the involvement of antibody.