Excretory/secretory antigen of Toxocara canis: recognition profiles of polyclonal and larvicidal monoclonal antibodies
- 1 March 1988
- journal article
- Published by Wiley in Parasite Immunology
- Vol. 10 (2) , 237-241
- https://doi.org/10.1111/j.1365-3024.1988.tb00217.x
Abstract
Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in-vitro microprecipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in-vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS-PAGE (177-77 kD, 43-15 kD) of immunoprecipitates of human and mouse positive polyclonal antisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177-77 kD range.Keywords
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