An Observation of Apparent Substitution of Pantothenate by Thiamine and Choline

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Abstract
A basal medium of vitamin-free acid-hydrolyzed casein medium did not support the growth of the Hall strain of Clostridium botulinum, type A. In expts. utilizing a minimal washed inoculum it was established that when one vitamin at a time was removed from a mixture supplementing the basal medium growth occurred except in the case when biotin was absent. However, the acid-hydrolyzed casein medium supplemented with biotin alone did not support growth. Biotin and Ca pantothenate supported slight growth. Thiamine, choline and biotin supported growth in the absence of pantothenate. A possible explanation of the results obtained is that the capacity of the organism to store pantothenic acid, thiamine, and choline is minimal so that when these materials are not provided by the external environment growth cannot be initiated. An external supply provides an initial source that can then be supplemented by synthesis during cell growth.

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