Purification of an alkaline phosphatase-lipoprotein-X complex.
Open Access
- 1 April 1980
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 26 (5) , 604-608
- https://doi.org/10.1093/clinchem/26.5.604
Abstract
An alkaline phosphatase isoenzyme was observed in an abnormal position in the agar-agarose gel pattern of sera from several patients suffering from intrahepatic and extrahepatic cholestasis. We purified this isoenzyme, by gel filtration and affinity chromatography, from the serum of a patient suffering from extrahepatic cholestasis. Analysis demonstrated an alkaline phosphatase-lipoprotein-X complex with a relative molecular mass of at least 669,000. We discuss the interpretation of alkaline phosphatase isoenzyme patterns produced by different techniques.Keywords
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