An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins.
- 1 March 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (5) , 1576-1580
- https://doi.org/10.1073/pnas.85.5.1576
Abstract
A fusion between tsr (encoding the inner membrane protein Tsr) and phoA (encoding the periplasmic protein alkaline phosphatase, AP) generates a membrane-bound hybrid protein (Tsr-AP 2) with AP enzymatic activity. The hybrid protein is proteolytically unstable and is broken down to yield a smaller, soluble species with AP activity. We devised a genetic screen to distinguish between cells containing only membrane-bound AP and those containing soluble AP. The screen depends on diffusion of soluble AP away from cells with a leaky outer membrane to produce a halo of AP activity around colonies on solid growth medium. Several mutants lacking this halo show reduced degradation of TSr-AP 2. One mutant is also defective in breakdown of five other abnormal periplasmic proteins but not of two cytoplasmic proteins. The mutation in this strain, degP4::Tn5, defines a locus distinct from previously identified loci that affect protein stability or protease activities. This strain may be useful for preventing the breakdown of unstable foreign proteins in Escherichia coli.This publication has 29 references indexed in Scilit:
- A Genetic Approach to Analyzing Membrane Protein TopologyScience, 1986
- TnphoA: a transposon probe for protein export signals.Proceedings of the National Academy of Sciences, 1985
- Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Protein degradation in Escherichia coli: the lon gene controls the stability of sulA protein.Proceedings of the National Academy of Sciences, 1983
- Control of phage λ development by stability and synthesis of cll protein: Role of the viral clll and host hflA, himA and himD genesCell, 1982
- The SOS regulatory system of Escherichia coliCell, 1982
- Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?Cell, 1981
- Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coliNature, 1980
- Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and φ80 transducing phagesJournal of Molecular Biology, 1975
- Mutants of Escherichia coli with a Defect in the Degradation of Nonsense FragmentsNature New Biology, 1973