Further Studies on the Nature and Source of the Conjugated Bile Pigments

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Abstract
Summary and conclusions Rat-fistula bile, isolated rat liver, liver slices incubated with bilirubin, and the hepatectomized dog were utilized to study the nature and sources of conjugated bile pigment. The technics of reverse-phase column chromatography, chemical-partition chromatography, and paper chromatography were employed and molar ratios were calculated. Probably pigment 1 is formed solely in extrahepatic sites. The evidence favors a bilirubin monoglucuronide structure for pigment 1 rather than a complex of bilirubin and pigment 2. In the dog, pigment 2 probably is formed only within the liver. It is suggested that bilirubin diglucuronide may be formed intrahepatically from bilirubin directly, as well as from part of the extrahepatic pigment 1.

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