Primary and secondary structure specificity of the cleavage of ‘single-stranded’ DNA by endo-nuclease HinfI
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (9) , 2763-2773
- https://doi.org/10.1093/nar/10.9.2763
Abstract
The interaction of endonuclease Hinf I with single-stranded fd DNA was examined, the sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and tested as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.Keywords
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