A Computerized Peptide Synthesizer with Feed Back Control.
- 1 January 1978
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 32b (10) , 703-713
- https://doi.org/10.3891/acta.chem.scand.32b-0703
Abstract
The feedback control in the automated system described is based on perchloric acid titration of free amino groups. If an unsatisfactory yield of coupling or deblocking is found, the coupling or deblocking may automatically be repeated. A complete printout of the synthesis is obtained. Two syntheses of leucine enkephalin are described, one with dicyclohexylcarbodiimide in deficiency. The products are characterized by amino acid analysis, high performance liquid chromatography and mass spectrometry. The system described is highly flexible regarding changes in the software as well as in the analytical, reactor and metering units. In principle, the basis construction of the synthesizer may be applied to solid phase synthesis of oligonucleotides.This publication has 4 references indexed in Scilit:
- The limits of reaction of radioactive dicyclohexylcarbodiimide with amino groups during solid-phase peptide synthesisThe Journal of Organic Chemistry, 1977
- N-Alkylation of Proline Residues by Chloromethyl Groups on the Resin in Solid-Phase Peptide SynthesisHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- A Computer-Controlled Peptide SynthesizerHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- Untersuchungen zur totalsynthese des ferredoxins—ITetrahedron, 1968