URACIL-DNA GLYCOSYLASE AND DEOXYURIDINE TRIPHOSPHATASE - STUDIES OF ACTIVITY AND SUBCELLULAR LOCATION IN HUMAN NORMAL AND MALIGNANT LYMPHOCYTES
- 1 January 1983
- journal article
- research article
- Vol. 43 (7) , 583-590
Abstract
Crude extracts of human lymphocytic cells contain 2 enzymes which putatively exclude uracil from DNA: uracil-DNA glycosylase and deoxyUTPase (dUTPase). Their activities were found in different types of benign and malignant cells: resting and mitogen-stimulated peripheral blood T- and B-lymphocytes, and in a lymphoblastic cell line with neither B- nor T-differentiation. The proliferative stage of the cell determined the dUTPase activity rather than the phenotypic origin of the population. This was demonstrated with peripheral blood lymphocytes: the activity of dUTPase in resting cells was very low and a 15- to 27-fold increase took place during mitogenic stimulation. Mitogens slightly enhanced the uracil-DNA glycosylase activity. The physiological roles of these enzymes may be connected with their subcellular location. The subcellular distributions of the enzymes were different; uracil-DNA glycosylase was most abundantly present near to cellular DNA, i.e., in nuclei, but dUTPase was nearly exclusively a cytoplasmic enzyme.This publication has 22 references indexed in Scilit:
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