Initiation of in vitro mRNA synthesis from the wild-type lac promoter.
- 1 November 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (11) , 4394-4398
- https://doi.org/10.1073/pnas.72.11.4394
Abstract
An in vitro transcription system, dependent on catabolite gene activator protein (CAP), utilizing a 200 base-pair restriction fragment, has been used to show that the initiation site of the wild-type Escherichia coli lac mRNA, and that of two mutants, 8d and ps, are identical to that previously reported for the CAP-independent promoter mutant UV5. Order of addition experiments are used to show that the binding of lac repressor to the operator is competitive with that of the RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to the promoter, thus demonstrating functional overlap of the operator and promoter sites.Keywords
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