DETECTION OF ANTILYMPHOCYTE ANTIBODIES IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS BY INDIRECT IMMUNOFLUORESCENCE ON ACETONE-FIXED LYMPHOCYTES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (2) , 240-249
Abstract
Antilymphocyte antibodies in normal and SLE [systemic lupus erythematosus] sera reacted with lymphocytes fixed in acetone at -30.degree. C for 10 min. At a dilution of 1:40, 3.7% of normal and 90.9% of SLE sera were positive with the use of indirect immunofluorescence [IIF] with a pepsin-digested, FITC[fluorescein isothiocyanate]-labeled anti-Fab serum. The reaction was independent of temperature between 4.degree. and 37.degree. C. Three patterns of staining were seen in the lymphocyte surface: reticular, ring and globular. The ring pattern appeared to correlate with Ig[immunoglobulin]M, and the reticular and globular patterns with IgG antilymphocyte antibodies. Absorption with lymphocytes, insolubilized extracts of rat liver and kidney, and human brain revealed that antilymphocyte and ANA [anti-nuclear antibody] activity could be independently removed. Variation in reactivity with cells from different normal donors was similar to that seen with the microcytotoxicity test. Acetone-fixed lymphocytes appear to be a much more sensitive target than viable cells in suspension in IIF tests for antilymphocyte antibodies. The IIF test with fixed cells also appears slightly more sensitive than cytotoxicity testing and has the advantage of allowing storage of target cells.

This publication has 0 references indexed in Scilit: