Preservation of Basidiospores of Laccaria laccata for Use as Mycorrhizal Inoculum
- 1 January 1975
- Vol. 67 (1) , 167-170
- https://doi.org/10.2307/3758239
Abstract
Fresh pilei of L. laccata were placed on glass sheets for 12-24 h to produce spore prints from which the basidiospores were scraped. Spores were placed in tear-drop lyophilization flasks and quick-frozen in liquid N, evacuated and hermetically sealed. Neither fresh nor lyophilized spores could be germinated on laboratory media although about 25% were viable as judged by staining. It was possible, however, to establish ectomycorrhizae in 46% of experimentally inoculated Douglas-fir seedlings.This publication has 0 references indexed in Scilit: