Localization with the Electron Microscope of p27 Rous Sarcoma Virus Internal Protein in Chicken Embryo Fibroblasts

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Abstract
Protein 76 (Pr76), the precursor of the so-called gag protein of avian leukosarcoma viruses, was localized in the light and electron microscope by using the immunoperoxidase technique in Rous sarcoma virus-infected chick embryo fibroblasts. The kinetics of the progression of the protein through the cell was studied at different times after infection. The protein was first found (after 8 h) on ribosomes located near the nucleus and then proceeded rapidly to the periphery of the cell. After 5-6 days, intracytoplasmic staining faded away and only the ribosomes close to the plasma membrane were stained. A possible association of a protein with the cytoskeleton is suggested.

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