Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude-mouse-passaged nasopharyngeal carcinoma
- 15 January 1979
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 23 (1) , 1-7
- https://doi.org/10.1002/ijc.2910230102
Abstract
After nude-mouse-passage, a carcinoma of the nasopharynx was found to contain a few scattered C-t/pe retrovirus particles. Culture of this nude-mouse-grown material over solid agar allowed the human tumour cells to grow as multicellular spheroids which increased in number by budding and could be subcultured for up to 6 months. Within the spheroids the human tumour cells expressed increased retrovirus replication and large numbers of C-type particles were observed. Treatment with a halogenated pyrimidine further enhanced the virus production. This tissue culture system provided sufficient virus production for the identification of the retrovirus as an endogenous xenotropic murine leukaemia virus and not a human agent.This publication has 16 references indexed in Scilit:
- Cytogenetics of malignant epithelial cells and lymphoblastoid cell lines from nasopharyngeal carcinomaBritish Journal of Cancer, 1978
- “sternberg‐reed” giant cells of hodgkin's disease: Cultivation in vitro, heterotransplantation, and characterization as neoplastic macrophagesInternational Journal of Cancer, 1977
- Epithelial Characteristics of Tumor Cells in Nasopharyngeal Carcinoma Passaged in Nude Mice: Ultrastructure 2JNCI Journal of the National Cancer Institute, 1976
- C-type virus particles in human tumours transplanted into nude miceBritish Journal of Cancer, 1976
- Cytogenetic studies on human lymphoblastoid cell lines from burkitt's lymphomas and other sourcesInternational Journal of Cancer, 1974
- Direct Evidence for the Presence of Epstein-Barr Virus DNA and Nuclear Antigen in Malignant Epithelial Cells from Patients with Poorly Differentiated Carcinoma of the NasopharynxProceedings of the National Academy of Sciences, 1974
- Activation of Leukemia Viruses by Graft-Versus-Host and Mixed Lymphocyte Reactions In VitroProceedings of the National Academy of Sciences, 1972
- Murine Leukemia Virus: High-Frequency Activation in vitro by 5-Iododeoxyuridine and 5-BromodeoxyuridineScience, 1971
- Plaque assay techniques for murine leukemia virusesVirology, 1970
- Nasopharyngeal carcinoma (NPC). I. Types of cultures derived from tumour biopsies and non‐tumorous tissues of chinese patients with special reference to lymphoblastoid transformationInternational Journal of Cancer, 1970