The invertase complement of cold-hardy and cold-sensitive wheat leaves

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Abstract
The invertase (EC 3.2.1.26) of leaves from two cultivars of wheat (Triticum aestivum L. emend Thell. ssp. vulgare), Rescue (cold-sensitive) and Kharkov 22 MC (cold-hardy), grown at 20 °C (non-hardening conditions) and at 4–6 °C (cold-hardening conditions), has been separated into three different forms by gel filtration. In Rescue wheat, the complement of different forms of invertase from the leaves did not change greatly when the plants were grown at different temperatures. In Kharkov 22 MC grown at 20 °C, the quantity of the medium-molecular-weight form of invertase was much greater than that of the high-molecular-weight form of invertase; however, when this variety was grown at 4–6 °C, the relative quantities of these two forms of invertase were reversed. This change in the ratio of the different forms of invertase in the leaves appears to be associated with the process of cold-hardening.

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