PLASMA CATECHOLAMINES VIA AN IMPROVED FLUORIMETRIC ASSAY - COMPARISON WITH AN ENZYMATIC METHOD

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (2) , 421-427
Abstract
The fluorimetric method of Renzini and Valori, utilizing alumina and ion exchange chromatography, was modified to include dl-3H norepinephrine (3H-NE) for individual plasma NE recoveries and mercaptoethanol as a stabilizing agent for the fluorescent lutines. Plasma NE and epinephrine (E) were measured in hypertensive patients, and results were compared with those of the enzymatic method of Engelman in duplicate plasma specimens. The correlations of the NE and the total plasma catecholamine concentrations determined by the 2 methods were borh r = 0.98 (P < 0.001) and that of the E concentrations was r = 0.79 (P < 0.01). The reproducibility of the method for measuring 10 mlp aliquots of pooled plasma yielded a SD of less than 10% of the mean. The fluorimetric method was more economical, less time-consuming and as reliable as the enzymatic method for quantitating plasma catecholamines.

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