Fertility of dairy cattle after artificial insemination with semen frozen in a lipoprotein diluent
- 1 September 1977
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 51 (1) , 175-177
- https://doi.org/10.1530/jrf.0.0510175
Abstract
A lipoprotein separated from egg yolk by centrifugation and column chromatography was used to prepare a diluent containing 2.9% trisodium citrate.cntdot.2H2O (pH 7.0) and 7% glycerol. Ejaculates from Friesian bulls were split between this and 2 control diluents containing egg yolk, glycerol and citrate or lactose and used to inseminate once 1782 unselected Friesian cows. By 16 wk after insemination with semen frozen in the egg yolk-lactose, egg yolk-citrate or lipoprotein-citrate diluents, 66.4, 67.1 and 66.1% of cows, respectively, had not returned to service.This publication has 5 references indexed in Scilit:
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- Gel filtration of native and modified pig serum lipoproteinsJournal of Chromatography A, 1968
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- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951