Anticodon‐dependent aminoacylation of RNA minisubstrate by lysyl‐tRNA synthetase
- 17 December 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 314 (3) , 256-258
- https://doi.org/10.1016/0014-5793(92)81483-3
Abstract
Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNALys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)20-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.Keywords
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