Functional Characterization of Brucella melitensis NorMI, an Efflux Pump Belonging to the Multidrug and Toxic Compound Extrusion Family
- 1 September 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (9) , 3050-3
- https://doi.org/10.1128/aac.46.9.3050-3053.2002
Abstract
Two putative proteins (NorMI and NorMII) similar to the multidrug efflux protein NorM of Vibrio parahaemolyticus are encoded by the Brucella melitensis 16 M genome. We show that a drug-hypersusceptible Escherichia coli strain overexpressing NorMI displays increased resistance to norfloxacin, ciprofloxacin, gentamicin, tetraphenylphosphonium ion, acriflavine, and berberine. This elevated resistance was proven to be mediated by an energy-dependent efflux mechanism. NorMI belongs to the multidrug and toxic compound extrusion family and is the first multidrug efflux protein identified in Brucella spp.Keywords
This publication has 27 references indexed in Scilit:
- VmrA, a Member of a Novel Class of Na + -Coupled Multidrug Efflux Pumps from Vibrio parahaemolyticusJournal of Bacteriology, 2002
- A MATE Family Multidrug Efflux Transporter Pumps out Fluoroquinolones in Bacteroides thetaiotaomicronAntimicrobial Agents and Chemotherapy, 2001
- Phylogeny of multidrug transportersSeminars in Cell & Developmental Biology, 2001
- Research lettersJournal of Tropical Pediatrics, 2001
- RpoN‐dependent transcription of rpoH?Molecular Microbiology, 1999
- Brucellosis: an OverviewEmerging Infectious Diseases, 1997
- Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone‐resistant isolatesMolecular Microbiology, 1994
- Development of ciprofloxacin resistance in Brucella melitensisJournal of Antimicrobial Chemotherapy, 1990
- Trimethoprim-Sulfamethoxazole in BrucellosisThe Journal of Infectious Diseases, 1973
- Human brucellosis: an evaluation of antibiotics in the treatment of brucellosisPostgraduate Medical Journal, 1967